Measuring Oxygen Consumption Rate in Caenorhabditis elegans.
نویسندگان
چکیده
The rate of oxygen consumption is a vital marker indicating cellular function during lifetime under normal or metabolically challenged conditions. It is used broadly to study mitochondrial function (Artal-Sanz and Tavernarakis, 2009; Palikaras et al., 2015; Ryu et al., 2016) or investigate factors mediating the switch from oxidative phosphorylation to aerobic glycolysis (Chen et al., 2015; Vander Heiden et al., 2009). In this protocol, we describe a method for the determination of oxygen consumption rates in the nematode Caenorhabditis elegans.
منابع مشابه
Apparent uncoupling of energy production and consumption in long-lived Clk mutants of Caenorhabditis elegans
Clk mutants of Caenorhabditis elegans are characterised by an overall slow down of temporal processes and increase in life span. It was hypothesised that Clk mutations slow down the pace of many cellular functions and lower the rate of energy metabolism, possibly resulting in slower production of reactive oxygen species which in turn could result in slower ageing. We tested this hypothesis by m...
متن کاملاستفاده از تعامل نماتد Caenorhabditis elegans، قارچ Arthrobotrys oligospora و باکتری Bacillus subtilis در کنترل نماتد Meloidogyne javanica
در این تحقیق از تعامل نماتد Caenorhabditis elegans، قارچ Arthrobotrys oligospora و باکتری Bacillus subtilis در کنترل نماتد مولد گره ریشه Meloidogyne javanica استفاده شد. باکتری مذکور جهت تحریک سیستم دفاعی گیاه در بدو تیمار و به عنوان غذای نماتد C. elegans و نماتد C. elegans به منظور افزایش تولید تله استفاده شد. قارچoligospora A. پس از 72 ساعتموجب مرگ و میر 77% لاروهای نماتد M. javanica گردید، ...
متن کاملBroad oxygen tolerance in the nematode Caenorhabditis elegans.
This study examined the effects of oxygen tensions ranging from 0 to 90 kPa on the metabolic rate (rate of carbon dioxide production), movement and survivorship of the free-living soil nematode Caenorhabditis elegans. C. elegans requires oxygen to develop and survive. However, it can maintain a normal metabolic rate at oxygen levels of 3.6 kPa and has near-normal metabolic rates at oxygen level...
متن کاملRole of oxygen consumption in hypoxia protection by translation factor depletion.
The reduction of protein synthesis has been associated with resistance to hypoxic cell death. Which components of the translation machinery control hypoxic sensitivity and the precise mechanism has not been systematically investigated, although a reduction in oxygen consumption has been widely assumed to be the mechanism. Using genetic reagents in Caenorhabditis elegans, we examined the effect ...
متن کاملDetermination of the effects of food preservatives benzoic acid and sodium nitrate on lifespan, fertility and physical growth in Caenorhabditis elegans
Presently, the use of protective food additives such as benzoic acid and sodium nitrate is quite common. However, it was found that these additives, which initially appeared to be harmless, led to the emergence of a number of health problems. Cancer and diseases and deaths with no apparent causes are among the leading concerns. Therefore, the studies which can reveal the genotoxic potential of ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Bio-protocol
دوره 6 23 شماره
صفحات -
تاریخ انتشار 2016